Journal:

Article Title: Peptide and Amino Acid Transporters Are Differentially Regulated during Seed Development and Germination in Faba Bean 1

doi: 10.1104/pp.103.024422

Figure Lengend Snippet: Analysis of transcript accumulation of VfPTR1 and VfAAP1 in different tissues and seed developmental stages of faba bean. Northern blots were hybridized with 32P-labeled cDNA probes of VfPTR1 (a), VfAAP1 (b), and ubiquitin (c) as loading control. Ten micrograms of total RNA isolated from different tissues and seed developmental stages was loaded per lane. dap, Days after pollination.

Article Snippet: Molecular Cloning and cDNA Library Screening RT-PCR was performed with an Oligo(dT) 23 primer (Sigma-Aldrich Chemie, Steinheim, Germany) and total RNA was extracted from developing cotyledons as template.

Techniques: Northern Blot, Labeling, Ubiquitin Proteomics, Control, Isolation

Journal:

Article Title: Peptide and Amino Acid Transporters Are Differentially Regulated during Seed Development and Germination in Faba Bean 1

doi: 10.1104/pp.103.024422

Figure Lengend Snippet: Analysis of transcript accumulation of VfPTR1, VfPTR2, and VfAAP1 in seedlings of faba bean harvested at different time points. Ten micrograms of total RNA extracted from different parts of the seedlings and at different time points was loaded per lane; i.e. before roots had ruptured the seed coats (4 dai), when roots were between 0.5 and 1.0 cm long (6 dai), and when roots were approximately 4.0 cm long (8 dai). Northern blots were hybridized with 32P-labeled cDNA probes of VfPTR1 (a), VfPTR2 (b), and VfAAP1 (c). An approximate size scale (kilobytes) is indicated. Equal loading between samples was confirmed by running an ethidium bromide-stained gel (data not shown).

Article Snippet: Molecular Cloning and cDNA Library Screening RT-PCR was performed with an Oligo(dT) 23 primer (Sigma-Aldrich Chemie, Steinheim, Germany) and total RNA was extracted from developing cotyledons as template.

Techniques: Northern Blot, Labeling, Staining